Pharmacology Research Group

1. Exploring Natural Antidepressants Using Behavioral And Enzyme Linked Immunosorbent Assays

Exploring natural antidepressants using behavioral and enzyme linked immunosorbent assays


Project Funding
 Funding Body Funds
1 ISESCO-COMSTECH Joint research Program no logo 10000 $


Project Members
  • Dr. Ghulam  Abbas
  • 2. Preclinical Evaluation Of Natural Substances For Anti-Depressant Potential Using Behavioral Assays

    Preclinical evaluation of natural substances for anti-depressant potential using behavioral assays

    Project Members
  • Dr. Ghulam  Abbas
  • 3. Exploring Genotoxic Potential Of Herbs Commonly Used In Pakistan

    Exploring Genotoxic Potential of Herbs Commonly Used in Pakistan

    Project Members
  • Dr. Ghulam  Abbas
  • 4. Providing Scientific Evidences To The Folkloric Antidepressants Of Pakistan

    Providing Scientific Evidences to the Folkloric Antidepressants of Pakistan

    Project Members
  • Dr. Ghulam  Abbas
  • 5. Exploring Systemic Toxicity Of The Herbs Commonly Used In Pakistan

    Phytotherapy is a very common practice in Pakistan. Literature survey exhibited lack of reports addressing toxicological actions of herbs frequently used in Pakistan. Considering this, the present study is designed to explore the systemic toxicity of herbs. To begin with, the local market, herbalists and industrial herbal products will be surveyed to identify the herbs used often by our peoples. The top three utilized herbs will be evaluated for their toxicological potential using repeated dose toxicity (food and water consumption, weight variation, hematology, blood chemistry and urinalysis) as per regulatory requirement by food and drug administration (FDA). The data obtained through this study will help protecting our peoples from the toxic effects of these herbs thereby improving their socio-economic status. Additionally, this study will reduce the burden on our health care system.

     

     


    Project Funding
     Funding Body Funds
    1 COMSTECH-TWAS no logo 15000 $


    Project Members
  • Dr. Ghulam  Abbas
  • 6. Pharmacological Investigation Of Medicinal Plants Available In Hazara Division And Related Northern Areas Based On Their Medicinal Use In Cardiovascular Disorders

    Since the beginning of human civilization, herbs have been remained an integral part of society, valued for both their culinary and medicinal properties. There is a great potential in the medicinal herbs to treat diseases, particularly those refracted to the modern medicine. World Health Organization, emphasize, particularly in the third world, where 80% of the population rely on tradition medicine, the utilization of indigenous medicinal plants in their health care system and their scientific evaluation and validation. As Hazara division and related northern areas are rich in medicinal plants and where use of traditional medicine is since time immemorial. Therefore, the Pharmacological evaluation of the local medicinal plants in order to explore the underlying mechanisms responsible for their use in the traditional medical system is the need of the time. Therefore, various plants used by the local community of Hazara division for the treatment of various cardiovascular disorders will be scientifically studied for their effects and the underlying mechanism(s). For this purpose, the plants will be selected on the basis of their Ethnopharmacological uses. The selected plants will be collected from their habitats. The shade-dried and pulverized plant material will then be subjected to extraction using suitable solvent(s). The resulting crude extracts will be phytochemically evaluated for various classes of constituents using microchemical tests and the HPLC chromatograms. Then these extracts will be screened for cardiovascular activities. The plant extracts showing good results in the preliminary pharmacological screening will be studied in detail especially the underlying mechanism(s). Moreover, the extracts will be fractionated using bioassay guided protocols and the subsequent fractions will be screened for the respective bioactivities and will also be chemically characterized. This investigation would be a valuable addition to the scientific literature of the proposed medicinal plants and may also possibly lead to identify some botanicals with combination of activities either with synergistic and or side effect neutralizing potential. 

    Project Members
  • Dr. Abdul Jabbar  Shah (Group Leader)
  • 7. Bio-Assay Guided Fractionation And Isolation Of Anti-Cancer Constituents From Otostegia Limbata

    Breast carcinoma is the second leading cause of death due to cancer among females. In Pakistan, it is more common at a young age contrary to the West where it is more common after 60 years. Many treatment options are available for this disease with major limitations of cost, effectiveness and safety. So, there is an urgent need to establish a cheap, safe and effective treatment protocol for breast cancer. Otostegia limbata (Lamiaceae) is a medicinally important plant used as folk medicine for different diseases including hypertension, inflammation, wounds and gum diseases. However, the literature lacks the scientific investigation of this plant for screening and isolation of chemical constituents for breast cancer activity. Therefore, the aim of current study is to determine the cytotoxic potential and isolation of various chemical constituents from O. limbata. For this purpose, O. limbata (leaves) will be subjected to extraction with methanol followed by bio-assay guided fractionation using solvent technique. The most active fraction(s) will be subjected to various chromatographic techniques for the isolation for various active chemical constituents. Brine-shrimp cytotoxicity and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays will be performed for the screening of cytotoxic potential of crude extract, fractions and bioactive chemical constituents of O. limbata.

    Project Funding
     Funding Body Funds
    1 COMSATS Institute of Infomation Technology no logo 200000 Rs


    Project Members
  • Dr. Nabi Shah
  • 8. In Vitro And In Vivo Evaluation Of The Antimicrobial And Antifungal Activity Of Equistem Arvence And Monotheca Buxifolia Toward Burn Healing.

    Burn injuries are very common and frequently occur in developing countries including Pakistan. In these areas peoples are bound to use traditional ointments. Scientific studies and researches have shown that there are some plants which can heal the wound injuries more effectively than other medicines. The quality of leads arising from natural products is better and is often more biologically friendly due to co-evolution with receptor sites of biological system. Equisetum arvense belonging to the family Equisetaceae is an herbaceous perennial plant, also known as the field horsetail and is distributed in the Naran Valley. Monotheca buxifolia is a monotypic genus of the family Sapotaceae, locally known as “Gurgura”. It is an evergreen fruit-producing medicinal tree, distributed in North-west Pakistan. In the present study the plants which will be screen out are Equisetum arvense and Monotheca buxifolia.These plants will be collected, shade dried and the plant will be extracted using different organic solvents. Each extract will be tested and bioactive part will be subjected to fractionation and chromatographic analysis and isolation. Beside these plant extracts and its subsequent fractions will be evaluated for potential pharmacological activities, antibacterial and antifungal activity with special reference to the burn wound healing.
    Project Members
  • Zia Rahman
  • 9. Fabrication Of A Three-Dimensional Tumor Microenvironment For Studying Effect Of Propitious Chemotherapeutics Agents From Natural Plants Origins.

    Cancer is a life-threatening human disease affecting millions of people throughout the world (Siddiqui N., https://www.shaukatkhanum.org.pk/news-a-events/events/228.html). Mostly cancer is diagnosed at later stages especially in developing countries like Pakistan and hence it causes death to millions of humans (Siddiqui N., https://www.shaukatkhanum.org.pk/news-a-events/events/228.html). In cancer certain cellular, molecular and genetic changes occur which leads to a loss of control on cell cycle and growth (Lu et al., 2006).Most of the cancer cases in Pakistan are reported at their late stage and hence remain untreatable. The current cancer therapies are extremely expensive and out of range for majority of the people in Pakistan. Therefore, there is an urgent need to find new, novel and less expensive way of treatment for this dangerous fatal disease. Pakistan is rich in various species of medicinal plants that are used in folk medicine. However, a large number of these medicinal plants have never been scientifically investigated. Previous results showed that many natural products extract, or bioactive chemical constituents have anti-cancer activities. Those reports encourage us to search local medicinal plants that are having beneficial effects for the treatment of cancer.  To perform this study, the selected plants will be collected from their habitat and will be shade-dried or will be purchased from an authentic herbal dealer. This will be followed by pulverization. The powdered plant material will be extracted with methanol. After drying in rotary evaporator, the resulting extracts will be tested for its toxicity assay against the primary cells obtained from various organs of developmental chicks. The toxicity will also be evaluated against the non-cancerous cell lines using trypan blue exclusion method or MTT assay. Based on these assays the non-toxic extracts will be selected and its anti-cancer activity will be evaluated against breast cancer cell lines. All of the experiment will be performed in 2D and newly established 3D system. 

    Project Members
  • Dr. Muhammad Ikram
  • Dr. Taous  Khan